Analysis of neurotransmitter catecholamines and related amines in human urine and serum by chromatography and capillary electrophoresis with 1, 3, 5, 7-tetramethyl-8-(N-hydroxysuccinimidyl propionic ester)-difluoro-boradiaza-s-indacene

Abstract Two sensitive and effective methods were developed for the detection of catecholamines related biogenic amines (dopamine, epinephrine, norepinephrine, serotonin, levodopa tyramine) using high performance liquid chromatography with fluorescence capillary electrophoresis laser-induced detection. A BODIPY fluorescent dye, 1, 3, 5, 7-tetramethyl-8-( N -hydroxysuccinimidyl propionic ester)-difluoroboradiaza- s-indacene was used as pre-column derivatization reagent. The separation conditions optimized in detail. In method, reaction completed at 35 °C 20 min. At wavelength λ ex / em = 493 nm/513 nm, dopamine, derivatives achieved baseline within 15 limits ( S/N 3) 1.0, 2.0, 5.0, 0.5 nmol/L, respectively. 25 Serotonin, tyramine dopamine reached 10 min 473 nm. tyramine, 0.3, 0.02, 0.2 amino compounds human serum urine samples detected successfully, recoveries 93.3%–106.7% 91.0%–103.1%,